Recombinant CD74 antibody

Catalog No. ABIN7072639

This Mouse Monoclonal antibody specifically detects CD74 in FACS, WB, IF, IHC, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant CD74 antibody (ABIN7072639)

Target: CD74 (HLA-DR-gamma (CD74))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: LN-2

Product Details anti-CD74 Antibody

Purpose: Anti-CD74 [LN-2], Mouse IgG1, kappa

Specificity: LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Cross-Reactivity: Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.

Isotype: IgG1 kappa

Application Details

Application Notes: Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells. Furthermore, this antibody was shown to be capable of distinguishing small cell lung cancer from a non small cell lung cancer as a part of a panel of antibodies (Ioachim et al., 1996). Furthermore, LN2 was successfully used in immunohistochemical and flow cytometric analysis of lung cancer tissue and cells (Ioachim et al., 1996). Finally, this antibody is also useful in immunofluorescence analysis of CD74, as it was very effective in labelling this protein in CD74-transfected COS-7 cells (Leng et al., 2003).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: Ia antigen, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, LN2

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints