Recombinant VCAM1 antibody

Catalog No. ABIN7072646

The Rabbit Chimeric anti-VCAM1 antibody has been validated for FACS and BP. It is suitable to detect VCAM1 in samples from Human.

Quick Overview for Recombinant VCAM1 antibody (ABIN7072646)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP)

Clone: B-K9 (C106-K9)

Product Details anti-VCAM1 Antibody

Purpose: Anti-CD106 [B-K9 (C106-K9)], Rabbit IgG, kappa

Specificity: B-K9 antibody recognizes CD106 (VCAM1). CD106 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing Balb/c mice against activated human umbilical vein endothelial cells (HUVEC).

Isotype: IgG kappa

Application Details

Application Notes: B-K9 antibody inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells, and thus it blocks the ability of white blood cells to attach themselves to the activated epithelium during, for instance, inflammation. Consequently, this antibody might be used in research where leukocytes adhesion-blocking is required (Uotila et al., 2014, Wagner et al. 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106

Background: VCAM1, Vascular cell adhesion protein 1, V-CAM 1, INCAM-100

UniProt: P19320

Pathways: Carbohydrate Homeostasis