Recombinant C5AR1 antibody (AA 1-31)

Catalog No. ABIN7072651

This Rabbit Chimeric antibody specifically detects C5AR1 in WB, FACS, ELISA, IP, Func and BP. It exhibits reactivity toward Human, Cow, Rabbit and Ferret.

Quick Overview for Recombinant C5AR1 antibody (AA 1-31) (ABIN7072651)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Cow, Rabbit, Ferret

Host: Rabbit

Clonality: Chimeric

Conjugate: This C5AR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Functional Studies (Func), Blocking Peptide (BP)

Clone: S5-1

Product Details anti-C5AR1 Antibody

Binding Specificity: AA 1-31

Purpose: Anti-C5aR [S5/1], Rabbit IgG, kappa

Specificity: S5/1 antibody recognizes the C5aR (CD88) protein, a 43 kDa receptor of C5a component of the complement cascade.

Cross-Reactivity: Cow, Ferret, Rabbit

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a synthetic peptide corresponding to C5a-R, amino acids: 1-31 (N terminal).

Isotype: IgG kappa

Application Details

Application Notes: S5/1 antibody inhibits C5a binding to its receptor C5aR (Oppermann et al., 1993). Furthermore, this antibody has been widely applied in the detection and analysis of C5aR via various methods. For instance, it was used in a flow cytometric study on peripheral blood lymphocytes where it was shown to bind to monocytes and granulocytes (Oppermann et al., 1993). It was also utilized in the Western blot experiments comparing C5aR expression in different groups of mice (Panayiotou et al., 2017). S5/1 was also successfull in labelling C5aR in the paraffin-embedded immunohistochemical samples of umbilical arteries (Fortin et al., 2002).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5aR

Background: CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR

UniProt: P21730

Pathways: Complement System