Recombinant CCR5 antibody (N-Term)

Catalog No. ABIN7072654

This Mouse Monoclonal antibody specifically detects CCR5 in WB, ELISA, FACS, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant CCR5 antibody (N-Term) (ABIN7072654)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: T21-8

Product Details anti-CCR5 Antibody

Binding Specificity: N-Term

Purpose: Anti-CCR5 [T21/8], Mouse IgG2a, kappa

Specificity:

T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.

Application Notes original Clone: T21/8 antibody is recommedned for the detection and analysis of CCR5 via various methods. For instance, one group used this antibody to detect intracellular localization of CCR5 in RBL-CCR5 cells via immunofluorescence staining (Pollok-Kopp et al., 2003). Another group investigated ligand-mediated down-modulation of CCR5 via flow cytometry, utilizing T21/8 antibody (Fox et al., 2015). With other anti-CCR5 antibodies which react with CCR5 phosphorylated on different serine residues (Ab01578, Ab01579), it can be used to study dynamic regulation of CCR5 and phosphorylation mechanisms.

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with intact RBL-CCR5 cells.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size