Recombinant CD74 antibody

Catalog No. ABIN7072682

The Mouse Monoclonal anti-CD74 antibody has been validated for FACS, WB, IP and RIA. It is suitable to detect CD74 in samples from Human.

Quick Overview for Recombinant CD74 antibody (ABIN7072682)

Target: CD74 (HLA-DR-gamma (CD74))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Clone: LL1 (EPB1)

Product Details anti-CD74 Antibody

Purpose: Anti-CD74 [LL1 (EPB1)], Mouse IgG1, kappa

Specificity: This antibody reacts with the cell surface epitope of the invariant chain (Ii) subunit of the immatrue MHC class-II antigen CD74.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation.

Isotype: IgG1 kappa

Application Details

Application Notes: Radioimmunobinding and flow cytometric analysis was used to analyse binding of LL1 to peripheral blood. LL1 did not react with T cell lines nor with normal diploid cells. The reactivity of LL1 (EB-1) to the tumor cells was checked using a live cell indirect immunoflurescence staining assay. LL1 stained positive for cell lines Raji (maxilla), Daudi (B-lymphoblast), RAMOS (b-lymphocyte), SU-DHL-7, ARH-77 (plasma cell leukemia), HS-Sultan (plasmacytoma). LL1 is reported to recognize most of the small and large lymphocytes of the germinal centre and no reactivity to marginal zone B-cells. It is also reported to react with interdigitating histiocytes of the T-cell zones. It was further reported that LL1 bound positively to Western blotted Raji cell antigens (PMID: 2663143). The specificity of LL1 for Ii subunit was determined by immunoprecipitation. Radiolabelled antibodies were used for internalization and catabolism of the antibody and it was seen that LL1 was internalized and catabolized very quickly as compared to some other antibodies like those which are specific for CD19, CD22 and anti-transferrin receptor. (PMID: 8947500). LL1 was evaluated to be a potential candidate for bone marrow imaging using Tcm labelled LL1 sulphur colloid imaging. (PMID: 9076770)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: Ii, p33, DHLAG, HLADG, Ia-GAMMAHLA, HLA Class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain,

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints