Recombinant CD80 antibody

Catalog No. ABIN7072690

This Rabbit Chimeric antibody specifically detects CD80 in ELISA and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD80 antibody (ABIN7072690)

Target: CD80

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD80 antibody is un-conjugated

Application: ELISA, Blocking Reagent (BR)

Clone: 16C10

Product Details anti-CD80 Antibody

Purpose: Anti-CD80 [16C10], Rabbit IgG, lambda

Specificity: This antibody binds human CD80 and inhibits the B7.1:CD28 pathway.

Characteristics:

Original Species of Ab: Rhesus Macaque

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.

Isotype: IgG lambda

Application Details

Application Notes: This antibody can be used for identification of human B7.1 antigen and is capable of inhibiting the binding of B7.1 to CD28, however it does not block the binding of B7.1 to CTLA-4. This antibody further inhibits the production of IL-2. Blocking of the primary activation signal between CD28 and B7.1 (CD80) with these antibodies while allowing the unimpaired or coincident interaction of CTLA-4 and B7.1 represents a combined antagonistic effect on positive co-stimulation with an agonistic effect on negative signaling. The binding of the antibody to soluble B7.1 was tested using ELISA. This antibody tested positive for binding to human C1q protein. This antibody is reported to cause keratinocyte hyperproliferation and decrease in psoriasis by inhibiting the B7:CD28 pathway. This antibody is further reported to be useful for the treatment of intestinal inflammation and rheumatoid arthritis.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: B7.1, CD28L, CD28LG, B7-1, B7, BB1, B7/BB1,T-lymphocyte activation antigen CD80, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1, CTLA4L, CTLA-4L

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints