Recombinant SARS-CoV-2 Spike antibody (C-Term, Extracellular Domain, RBD)

Catalog No. ABIN7072712

The Mouse Chimeric anti-SARS-CoV-2 Spike antibody is suitable to detect SARS-CoV-2 Spike in samples from SARS Coronavirus-2 (SARS-CoV-2). It has been validated for ELISA, Inh and GCI.

Quick Overview for Recombinant SARS-CoV-2 Spike antibody (C-Term, Extracellular Domain, RBD) (ABIN7072712)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Mouse

Clonality: Chimeric

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Inhibition (Inh), Grating Coupled Interferometry (GCI)

Clone: Sb#14

Product Details anti-SARS-CoV-2 Spike Antibody

Binding Specificity: C-Term, Extracellular Domain, RBD

Purpose: Anti-Spike protein (RBD) [Sb#14], Mouse IgG1-Fc Fusion,

Specificity:

This antibody recognizes and binds the SARS CoV 2 C-terminal receptor binding domain (RBD) located in the extracellular portion of the spike protein.

Application Notes original Clone: This antibody is recommended for detection of SARS CoV 2 or 2019-nCoV. Its high specificity to the novel coronavirus SARS-Cov-2 was confirmed via ELISA testing (Walter et al., 2020). Furthermore, grating-coupled interferometry demonstrated that this antibody binds the receptor binding domain (RBD) of the spike protein with high affinity (Walter et al., 2020). Subsequent testing showed that this clone exhibits exceptionally strong inhibition of binding of SARS-Cov-2 RBD to hACE2, which is the receptor for the virus (signal of RBD association with hACE2 decreased over 90 % ) (Walter et al., 2020).

Characteristics: This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Purification: Purified antibody.

Immunogen: This clone was originally isolated in a form of a synthetic nanobody (sybody) via a 'target swap' selection procedure against RBD-vYFP using ribosomal display and against RBD-Fc fusion during phage display rounds.

Isotype: IgG1-Fc Fusion

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Dromedary

OriginalFormat: VHH

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-CoV2 Spike protein

Target Type: Viral Protein

Background: Receptor Binding Domain, SARS CoV 2 S glycoprotein, Sb45, COVID-19 Spike protein, RBD, Receptor Binding Domain, E2 glycoprotein, E2, Human coronavirus 2 spike glycoprotein, Peplomer protein, S glycoprotein, SARS coronavirus 2 S protein, SARS coronavirus 2 Spike Protein, SARS CoV 2 Spike protein, SARS CoV 2, SARS-CoV-2 S protein, SARSCoV2, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe acute respiratory syndrome 2 spike glycoprotein, Severe acute respiratory syndrome virus 2 spike glycoprotein, Spike glycoprotein, 2019-nCoV, SARS-CoV2

UniProt: P0DTC2