Recombinant Mesothelin antibody

Catalog No. ABIN7072728

The Rabbit Chimeric anti-Mesothelin antibody (Clone 11-25) (ABIN7072728) specifically detects Mesothelin in ELISA, FACS and IP. The antibody is reactive with Human samples.

Quick Overview for Recombinant Mesothelin antibody (ABIN7072728)

Target: Mesothelin (MSLN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 11-25

Product Details anti-Mesothelin Antibody

Purpose: Anti-Mesothelin [11-25], Rabbit IgG, kappa

Specificity: This antibody binds the human mesothelin protein which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by intraperitoneally immunizing BALB/c mice with the purified recombinant mesothelin protein.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is recommended to be used in combination with mAb IC14-30 for detecting mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11-25 ) or an isotype-matched control antibody and followed by PE-conjugate anti mouse IgG. Antigen expression was detected by flow cytometry (PMID: 18394747). 64Cu labelled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (PMID: 25883990).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process