Recombinant Mesothelin antibody

Catalog No. ABIN7072729

This Mouse Monoclonal antibody specifically detects Mesothelin in ELISA, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant Mesothelin antibody (ABIN7072729)

Target: Mesothelin (MSLN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 11-25

Product Details anti-Mesothelin Antibody

Purpose: Anti-Mesothelin [11-25], Mouse IgG2b, kappa

Specificity: This antibody binds the human mesothelin protein which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by intraperitoneally immunizing BALB/c mice with the purified recombinant mesothelin protein.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody is recommended to be used in combination with mAb IC14-30 for detecting mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11-25 ) or an isotype-matched control antibody and followed by PE-conjugate anti mouse IgG. Antigen expression was detected by flow cytometry (PMID: 18394747). 64Cu labelled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (PMID: 25883990).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process