UCHL1 antibody (AA 35-140)

Catalog No. ABIN704396

The Rabbit Polyclonal anti-UCHL1 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect UCHL1 in samples from Human, Rat, Mouse, Cow, Goat and Chicken. There are 4+ publications available.

Quick Overview for UCHL1 antibody (AA 35-140) (ABIN704396)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse, Cow, Goat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCHL1 Antibody

Binding Specificity: AA 35-140

Cross-Reactivity: Chicken, Cow, Goat, Human, Mouse, Rat

Predicted Reactivity: Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PGP9.5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-UCHL1 antibody (ABIN704396)

Zheng, Zhu, Mu, Wu, Song, Zhai, Peng, Li, Hua: "CD49f promotes proliferation of male dairy goat germline stem cells." in: Cell proliferation, Vol. 49, Issue 1, pp. 27-35, (2016) (PubMed).

Li, Zhuang, Wu, Niu, Zhang, Li, Wei, Li, Hua: "Bovine male germline stem-like cells cultured in serum- and feeder-free medium." in: Cytotechnology, Vol. 68, Issue 5, pp. 2145-57, (2016) (PubMed).

Deng, Chen, Wang, Zhang, Tang, Li, Wang, Cheng, Jin, Li, Zhang, Yu, Lian, Liu, Liu: "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." in: Journal of pineal research, Vol. 60, Issue 4, pp. 435-47, (2016) (PubMed).

Zheng, Zhai, Li, Ma, Zhu, Du, Li, Hua: "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." in: Scientific reports, Vol. 6, pp. 37414, (2016) (PubMed).

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1/PGP9.5

Background:

Synonyms: NDGOA, PARK5, PGP95, PGP9.5, Uch-L1, HEL-117, PGP 9.5, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1, UCHL1

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour