eGFP antibody

Catalog No. ABIN7073829

The Rabbit Polyclonal anti-eGFP antibody has been validated for WB, IHC and IF. It is suitable to detect eGFP in samples from Tag.

Quick Overview for eGFP antibody (ABIN7073829)

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reactivity: Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eGFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-eGFP Antibody

Purification: Affinity purification

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse eGFP

Application Details

Application Notes: WB (Tag) 1:500-1:1000, IHC/IF (/) 1: 500-1: 1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for eGFP

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Alternative Name: EGFP

Background: Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

Molecular Weight: 29 kDa