EIF2AK1 antibody

Catalog No. ABIN7073875

The Rabbit Polyclonal anti-EIF2AK1 antibody (ABIN7073875) specifically detects EIF2AK1 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EIF2AK1 antibody (ABIN7073875)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF2AK1 Antibody

Cross-Reactivity: Human, Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse Heme-regulated inhibitor

Application Details

Application Notes: WB (H,M,R) 1 :500-1: 1000, IHC/IF (M,R) 1:800-1:1600/1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: Heme-regulated inhibitor

Background: The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.

Molecular Weight: 70 kDa

Gene ID: 15467

NCBI Accession: NP_038585

UniProt: Q9Z2R9

Pathways: Hepatitis C