Oct-4BOCT4B-190NT (AA 201-300) antibody

Catalog No. ABIN704531

Product Details

Target: Oct-4BOCT4B-190NT

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human OCT4B-190

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Liu, Huang, Yang, Wei: "OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation." in: BioMed research international, Vol. 2017, pp. 2756891, (2018) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Tanabe, Deguchi, Sato, Nemoto, Maruo, Madarame, Shida, Naya, Ogihara, Sahara: "Radioresistance of cancer stem-like cell derived from canine tumours." in: Veterinary and comparative oncology, (2014) (PubMed).

Darabi, Tiraihi, Ruintan, Abbaszadeh, Delshad, Taheri: "Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure." in: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 8, pp. 638-52, (2013) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).

Target Details

Target: Oct-4BOCT4B-190NT

Background:

Synonyms: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, POU5F1

Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Gene ID: 5460

UniProt: Q01860