LAT antibody

Catalog No. ABIN7074502

The Rabbit Polyclonal anti-LAT antibody (ABIN7074502) specifically detects LAT in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LAT antibody (ABIN7074502)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LAT Antibody

Cross-Reactivity: Human, Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse LAT

Application Details

Application Notes: WB (M,R) 1:500-1:1000, IHC/IF (H,M,R) 1:1000-1:4000/1:500-1:3000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background: Linker for activation of T-cells family member 1 (LAT) is a single-pass type III membrane protein which is required for TCR (T-cell antigen receptor) and pre-TCR-mediated signaling, both in mature T-cells and during their development This protein is involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Functionally T cell receptor (TCR) activation results in tyrosine phosphorylation of LAT, leading to the direct interaction with several proteins, including PLC-gamma 1, Grb2 and Gads. These direct ligands then mediate the indirect interaction of LAT with proteins such as SLP-76, Vav1 and Itk. PLC-gamma 1, Vav1 and Itk contain pleckstrin homology (PH) domains that interact with the enzymatic product of phosphoinositide-3-kinase (PI3k).

Molecular Weight: 38 kDa

Gene ID: 16797

NCBI Accession: NP_034819

UniProt: O54957

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway