CD40 Ligand antibody (iFluor™488)

Catalog No. ABIN7076852

The iFluor™488-conjugated Mouse Monoclonal anti-CD40 Ligand antibody (Clone 5C8) (ABIN7076852) specifically detects CD40 Ligand in FACS. The antibody is reactive with Human samples.

Quick Overview for CD40 Ligand antibody (iFluor™488) (ABIN7076852)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to iFluor™488

Application: Flow Cytometry (FACS)

Grade: GMP Grade

Clone: 5C8

Product Details anti-CD40 Ligand Antibody (iFluor™488)

Purpose: CD154 iFluor 488 Antibody

Characteristics: The clone 5C8, a mouse monoclonal antibody, specifically recognizes a ~30-33 kDa activation-induced human CD4+ T cells surface molecule known as CD154 or CD40 ligand (CD40L). The CD40 interacts with CD154 through T cell-B cell activating molecule, gp39, TNF cytokine related activation protein. CD154 is primarily expressed on the surface of activated CD4+ T cells but can also be expressed by platelets, mast cells, macrophages, basophils, NK cells, B cells, CD8+ T cells as well as non-hematopoietic cells including smooth muscle cells, endothelial cells, and epithelial cells. CD154 plays important roles in the pathogenesis of certain blood cancer such as the CD4+ T cells of patients with CLL fail to express surface CD154 antigen even after CD3 ligation, and proximal and distal activation events are required for the neonatal CD4+ T cells for the CD154 gene transcription to establish the normal immune system.

Purification: Purified

Purity: >95 %

Immunogen: Human D1.1 T cell line

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2, 0.2 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C, Conjugated antibodies should never be frozen.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154

Gene ID: 595

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints