DDX4 antibody (C-Term)

Catalog No. ABIN7091031

This Rabbit Polyclonal antibody specifically detects DDX4 in WB, ELISA and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for DDX4 antibody (C-Term) (ABIN7091031)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDX4 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DDX4 C-term

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4

Background:

Synonyms: DDX 4, DEAD (Asp Glu Ala Asp) box polypeptide 4, Dead box protein 4, Dead-box protein 4, MGC111074, MVH, Probable ATP dependent RNA helicase DDX4, VASA, VASA homolog, DDX4_HUMAN.

Background: ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).

Gene ID: 54514

UniProt: Q9NQI0