LEF1 antibody (AA 95-283)

Catalog No. ABIN7091338

The Mouse Monoclonal anti-LEF1 antibody has been validated for WB. It is suitable to detect LEF1 in samples from Human.

Quick Overview for LEF1 antibody (AA 95-283) (ABIN7091338)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: F11A3

Product Details anti-LEF1 Antibody

Binding Specificity: AA 95-283

Predicted Reactivity: Human

Immunogen: Recombinant human LEF-1 between 95-283 amino acids.

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background:

Synonyms: lymphoid enhancing factor-1, Transcription factor T cell specific 1 alpha , DKFZp586H0919, LEF 1, LEF1, Lymphoid enhancer binding factor 1, T cell specific transcription factor 1 alpha, TCF 1 alpha, TCF1 alpha, TCF1alpha, TCF1-alpha, LEF1_HUMAN, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha.

Background: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding