Pol II antibody
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- Target
- Pol II
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunofluorescence (IF), Western Blotting (WB)
- Cross-Reactivity
- Human
- Purification
- Purified by Protein G.
- Immunogen
- Monoclonal antibody raised in mouse against the YSPTSPS repeat in the B1 subunit of RNA polymerase II
- Isotype
- IgG1
-
- Application Notes
-
WB 1:300-5000
IF() - Restrictions
- For Research Use only
-
- Concentration
- 1 μg/μL
- Buffer
- PBS with 0.05 % sodium azide.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
-
- Target
- Pol II
- Synonyms
- 152117_at antibody, BG:DS00941.10 antibody, CG7885 antibody, D-rpII33 antibody, Dmel\\CG7885 antibody, Pol II antibody, Pol II RPII33 antibody, PolII antibody, PolIIo antibody, RNA Pol II antibody, RNA pol II antibody, RNA polII antibody, RNAP antibody, RPB3 antibody, Rbp3 antibody, Rpb3 antibody, Rpll33 antibody, br17 antibody, l(2)34Dg antibody, l(2)br17 antibody, l(2)k05605 antibody, l34Dg antibody, pol II antibody, polII antibody, rpII33 antibody, CG3180 antibody, DmRP140 antibody, Dmel\\CG3180 antibody, H5 antibody, II antibody, Pol IIc antibody, PolIIc antibody, RNA Pol II 140 antibody, RNA Pol IIc antibody, RNAP II antibody, RNApolII antibody, RP140 antibody, RPB2_DROME antibody, RPII140 antibody, RpII140[wimp] antibody, Rpb2 antibody, Rpll140 antibody, l(3)RplII140 antibody, rpII140/wimp antibody, wimp antibody, CG3284 antibody, Dmel\\CG3284 antibody, Pol-II antibody, RPII15 antibody, RpII215 antibody, dRPB9 antibody, l(3)88Be antibody, l(3)Z23 antibody, BcDNA:RH21608 antibody, CG1163 antibody, Dm6 antibody, Dmel\\CG1163 antibody, RPB6_DROME antibody, RPII18 antibody, RpABC14 antibody, Rpll18 antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II 33kD subunit antibody, RNA polymerase II 140kD subunit antibody, RNA polymerase II 15kD subunit antibody, RNA polymerase II 18kD subunit antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, RpII33 antibody, RpII140 antibody, RpII15 antibody, RpII18 antibody
- Background
-
Synonyms: POLR2A, RPB1, POLR2, RPOL2
Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression
- Gene ID
- 5430
- UniProt
- P24928
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