RPE65 antibody

Catalog No. ABIN7091644

The Rabbit Polyclonal anti-RPE65 antibody has been validated for WB and IHC (p). It is suitable to detect RPE65 in samples from Mouse.

Quick Overview for RPE65 antibody (ABIN7091644)

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPE65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RPE65 Antibody

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse RPE65

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPE65

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: RPE65

Background:

Synonyms: All-trans-retinyl-palmitate hydrolase, LCA 2, LCA2, Leber congenital amaurosis, mRPE 65, mRPE65, p63, rd 12, rd12, Retinal pigment epithelium specific 61 kDa protein, Retinal pigment epithelium specific 65 kDa protein, Retinal pigment epithelium specific protein, Retinal pigment epithelium specific protein 65 kDa, Retinal pigment epithelium-specific 65 kDa protein, Retinitis pigmentosa 20, Retinoid isomerohydrolase, RP 20, RP20, RPE 65, RPE65, RPE65_HUMAN, sRPE 65, sRPE65.

Background: The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65 kDa protein belonging to the _-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2.

Gene ID: 19892

UniProt: Q91ZQ5