VCP antibody

Catalog No. ABIN7091810

This anti-VCP antibody is a Mouse Monoclonal antibody detecting VCP in WB, IF, IHC (p), ICC and IHC (fro). Suitable for Human.

Quick Overview for VCP antibody (ABIN7091810)

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: N5A9

Product Details anti-VCP Antibody

Predicted Reactivity: Human,Mouse

Immunogen: Recombinant human VCP.

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF()
ICC 1:100-500

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: VCP

Background:

Synonyms: valosin-containing protein, 15S Mg(2+) ATPase p97 subunit, ATPase p97, IBMPFD, MGC131997, MGC148092, MGC8560, p97, TER ATPase, TERA, transitional endoplasmic reticulum ATPase, valosin-containing protein, yeast Cdc48p homolog, Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, p97, TERA_HUMAN.

Background: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway