NCK2 antibody (AA 301-380) (HRP)

Catalog No. ABIN706340

The Rabbit Polyclonal anti-NCK2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect NCK2 in samples from Mouse.

Quick Overview for NCK2 antibody (AA 301-380) (HRP) (ABIN706340)

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NCK2 Antibody (HRP)

Binding Specificity: AA 301-380

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCK2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Alternative Name: NCK2

Background:

Synonyms: Cytoplasmic protein NCK2, NCK2, GRB4, Growth factor receptor bound protein 4, NCK adaptor protein 2, Nck-2, NCK2, NCK2_HUMAN, NCKbeta, Non-catalytic region of tyrosine kinase adaptor protein 2, Noncatalytic region of tyrosine kinase beta, SH2/SH3 adaptor protein NCK-beta, SH2/SH3 adaptor protein NCK-beta.

Background: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Gene ID: 8440

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization