CD27 antibody (AA 20-191) (Biotin)

Catalog No. ABIN7093003

The Rabbit Monoclonal anti-CD27 antibody is suitable to detect CD27 in samples from Human. It has been validated for FACS and ELISA.

Quick Overview for CD27 antibody (AA 20-191) (Biotin) (ABIN7093003)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD27 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: DM57

Product Details anti-CD27 Antibody (Biotin)

Binding Specificity: AA 20-191

Purpose: Biotinylated Anti-CD27 antibody(DM57), Rabbit mAb

Purification: Purified from cell culture supernatant by affinity chromatography

Immunogen: Recombinant human CD27 (Ala20-Arg191) (ABIN6961160) produced by using human HEK293 cells

Isotype: IgG

Application Details

Application Notes: ELISA 1:5000-10000, Flow Cyt 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: Synonyms:CD27, TNFRSF7, S152, T14, Tp55
Description:The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Molecular Weight: 29 kDa

Gene ID: 939

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints