AKAP8L antibody

Catalog No. ABIN7111158

The Rabbit Polyclonal anti-AKAP8L antibody (ABIN7111158) specifically detects AKAP8L in WB, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for AKAP8L antibody (ABIN7111158)

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP8L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-AKAP8L Antibody

Purpose: AKAP8L antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: A kinase(PRKA) anchor protein 8-like

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IF: 1:20-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for AKAP8L

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Alternative Name: AKAP8L

Background:

Synonyms: A-kinase anchor protein 8-like (AKAP8-like protein)|Helicase A-binding protein 95 (HAP95)|Homologous to AKAP95 protein (HA95)|Neighbor of A-kinase-anchoring protein 95 (Neighbor of AKAP95)|AKAP8L|NAKAP|NAKAP95

Background: Could play a role in constitutive transport element(CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export(PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA(PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis(PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta(PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3, in this function seems to act redundantly with AKAP8(PubMed:16980585). May be involved in regulation of pre-mRNA splicing(PubMed:17594903). (Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters(PubMed:11884601). Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses(PubMed:11402034). In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription, in vitro negatively regulates DHX9 annealing activity(PubMed:25034436).

Molecular Weight: 72 kDa

Gene ID: 26993

UniProt: Q9ULX6

Pathways: SARS-CoV-2 Protein Interactome