ADHFE1 antibody

Catalog No. ABIN7111442

The Rabbit Polyclonal anti-ADHFE1 antibody is suitable to detect ADHFE1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC, WB and IP.

Quick Overview for ADHFE1 antibody (ABIN7111442)

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADHFE1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ADHFE1 Antibody

Purpose: ADHFE1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: alcohol dehydrogenase, iron containing, 1

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000, IHC: 1:20-1:200, IP: 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ADHFE1

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Alternative Name: ADHFE1

Background:

Synonyms: Hydroxyacid-oxoacid transhydrogenase, mitochondrial (HOT)|Alcohol dehydrogenase iron-containing protein 1 (ADHFe1)|Fe-containing alcohol dehydrogenase|ADHFE1

Background: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate(GHB) to succinic semialdehyde(SSA) coupled to reduction of 2-ketoglutarate(2-KG) to D-2-hydroxyglutarate(D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate(L-3-OHB) are also substrates for HOT with 10-fold lower activities.

Molecular Weight: 50 kDa

Gene ID: 137872

UniProt: Q8IWW8