ATG12 antibody

Catalog No. ABIN7111887

The Rabbit Polyclonal anti-ATG12 antibody (ABIN7111887) specifically detects ATG12 in WB, IHC, ELISA and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for ATG12 antibody (ABIN7111887)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-ATG12 Antibody

Purpose: ATG12 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ATG12 autophagy related 12 homolog

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:2000 , IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background:

Synonyms: Ubiquitin-like protein ATG12|Autophagy-related protein 12 (APG12-like)|ATG12|APG12|APG12L

Background: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.

Molecular Weight: 48-50 kDa

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy