ATG4B antibody

Catalog No. ABIN7111893

This anti-ATG4B antibody is a Rabbit Polyclonal antibody detecting ATG4B in WB, ELISA, IHC and IP. Suitable for Human, Mouse and Rat.

Quick Overview for ATG4B antibody (ABIN7111893)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ATG4B Antibody

Purpose: ATG4B antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ATG4 autophagy related 4 homolog B

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:2000 , IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background:

Synonyms: Cysteine protease ATG4B|AUT-like 1 cysteine endopeptidase|Autophagy-related cysteine endopeptidase 1 (Autophagin-1)|Autophagy-related protein 4 homolog B (HsAPG4B, hAPG4B)|ATG4B|APG4B|AUTL1|KIAA0943

Background: Cysteine protease required for the cytoplasm to vacuole transport(Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine(PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Molecular Weight: 44 kDa

Gene ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagy