BMI1 antibody (AA 1-326)

Catalog No. ABIN7112015

The Mouse Monoclonal anti-BMI1 antibody has been validated for WB, IHC, ELISA, IF and IP. It is suitable to detect BMI1 in samples from Human and Mouse.

Quick Overview for BMI1 antibody (AA 1-326) (ABIN7112015)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 8D5

Product Details anti-BMI1 Antibody

Binding Specificity: AA 1-326

Purpose: BMI1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-326aa

Immunogen: BMI1 polycomb ring finger oncogene

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background:

Synonyms: Polycomb complex protein BMI-1|Polycomb group RING finger protein 4|RING finger protein 51|BMI1|PCGF4|RNF51

Background: Component of a Polycomb group(PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Molecular Weight: 37 kDa

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy