CHST14 antibody

Catalog No. ABIN7112278

This Rabbit Polyclonal antibody specifically detects CHST14 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHST14 antibody (ABIN7112278)

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CHST14 Antibody

Purpose: CHST14 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: carbohydrate(N-acetylgalactosamine 4-0) sulfotransferase 14

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CHST14

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Alternative Name: CHST14

Background:

Synonyms: Carbohydrate sulfotransferase 14|Dermatan 4-sulfotransferase 1 (D4ST-1, hD4ST1)|CHST14|D4ST1

Background: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine(GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid(IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in-IdoUA-GalNAc-IdoUA-than in-GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development.

Molecular Weight: 45-50 kDa

Gene ID: 113189

UniProt: Q8NCH0

Pathways: Glycosaminoglycan Metabolic Process