Caspase 3 antibody (AA 29-175)

Catalog No. ABIN7112361

This anti-Caspase 3 antibody is a Mouse Monoclonal antibody detecting Caspase 3 in WB, IHC, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Caspase 3 antibody (AA 29-175) (ABIN7112361)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 2G7

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 29-175

Purpose: CASP3 antibody

Purification: protein A+G purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 29-175aa

Immunogen: caspase 3, apoptosis-related cysteine peptidase

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:300, IF: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background:

Synonyms: Caspase-3 (CASP-3)|Apopain|Cysteine protease CPP32 (CPP-32)|Protein Yama|SREBP cleavage activity 1 (SCA-1)|Caspase-3 subunit p17|Caspase-3 subunit p12|CASP3|CPP32

Background: Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Molecular Weight: 32-35 kDa, 19 kDa, 17 kDa

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation