CTCFL antibody (AA 361-663)

Catalog No. ABIN7112427

The Mouse Monoclonal anti-CTCFL antibody (Clone 2B10) (ABIN7112427) specifically detects CTCFL in WB, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for CTCFL antibody (AA 361-663) (ABIN7112427)

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTCFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 2B10

Product Details anti-CTCFL Antibody

Binding Specificity: AA 361-663

Purpose: CTCFL antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 361-663aa

Immunogen: CCCTC-binding factor(zinc finger protein)-like

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CTCFL

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Alternative Name: CTCFL

Background:

Synonyms: Transcriptional repressor CTCFL|Brother of the regulator of imprinted sites|CCCTC-binding factor|CTCF paralog|CTCF-like protein|Cancer/testis antigen 27 (CT27)|Zinc finger protein CTCF-T|CTCFL|BORIS

Background: CTCFL, also named BORIS(for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region(ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Molecular Weight: 75 kDa

Gene ID: 140690

UniProt: Q8NI51