CD14 antibody (AA 1-375)

Catalog No. ABIN7112444

The Mouse Monoclonal anti-CD14 antibody has been validated for WB and ELISA. It is suitable to detect CD14 in samples from Human and Mouse.

Quick Overview for CD14 antibody (AA 1-375) (ABIN7112444)

Target: CD14

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 0G10

Product Details anti-CD14 Antibody

Binding Specificity: AA 1-375

Purpose: CD14 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-375aa

Immunogen: CD14 molecule

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Monocyte differentiation antigen CD14|Myeloid cell-specific leucine-rich glycoprotein|Monocyte differentiation antigen CD14, urinary form|Monocyte differentiation antigen CD14, membrane-bound form|CD14

Background: Coreceptor for bacterial lipopolysaccharide(PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide(LPS)(PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response(PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway(PubMed:16880211). Binds electronegative LDL(LDL(-)) and mediates the cytokine release induced by LDL(-)(PubMed:23880187).

Molecular Weight: 50-55 kDa

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades