CHAF1A antibody

Catalog No. ABIN7112742

This anti-CHAF1A antibody is a Rabbit Polyclonal antibody detecting CHAF1A in WB, ELISA, IP, IHC and IF. Suitable for Human and Mouse.

Quick Overview for CHAF1A antibody (ABIN7112742)

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CHAF1A Antibody

Purpose: CHAF1A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: chromatin assembly factor 1, subunit A(p150)

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:4000, IP: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CHAF1A

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Alternative Name: CHAF1A

Background:

Synonyms: Chromatin assembly factor 1 subunit A (CAF-1 subunit A)|Chromatin assembly factor I p150 subunit (CAF-I 150 kDa subunit, CAF-I p150, hp150)|CHAF1A|CAF|CAF1P150

Background: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci(By similarity).

Molecular Weight: 150 kDa

Gene ID: 10036

UniProt: Q13111