Dihydrofolate Reductase antibody

Catalog No. ABIN7113482

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody is suitable to detect Dihydrofolate Reductase in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for Dihydrofolate Reductase antibody (ABIN7113482)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Dihydrofolate Reductase Antibody

Purpose: DHFR antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: dihydrofolate reductase

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background:

Synonyms: Dihydrofolate reductase|DHFR

Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

Molecular Weight: 21 kDa

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases