EMG1 antibody

Catalog No. ABIN7113791

The Rabbit Polyclonal anti-EMG1 antibody is suitable to detect EMG1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for EMG1 antibody (ABIN7113791)

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EMG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-EMG1 Antibody

Purpose: EMG1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: EMG1 nucleolar protein homolog

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EMG1

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Alternative Name: EMG1

Background:

Synonyms: Ribosomal RNA small subunit methyltransferase NEP1|18S rRNA (pseudouridine(1248)-N1)-methyltransferase|18S rRNA Psi1248 methyltransferase|Nucleolar protein EMG1 homolog|Protein C2f|Ribosome biogenesis protein NEP1|EMG1|C2F

Background: S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248(Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine(m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position(PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes(By similarity).

Molecular Weight: 27 kDa

Gene ID: 10436

UniProt: Q92979