EIF2AK1 antibody (AA 1-194)

Catalog No. ABIN7113933

This anti-EIF2AK1 antibody is a Rabbit Polyclonal antibody detecting EIF2AK1 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for EIF2AK1 antibody (AA 1-194) (ABIN7113933)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-EIF2AK1 Antibody

Binding Specificity: AA 1-194

Purpose: EIF2AK1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-194aa

Immunogen: eukaryotic translation initiation factor 2-alpha kinase 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: EIF2AK1

Background:

Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 1|Heme-controlled repressor (HCR)|Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase|Heme-regulated inhibitor (hHRI)|Hemin-sensitive initiation factor 2-alpha kinase|EIF2AK1|HRI|KIAA1369

Background: Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells(RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions(By similarity).

Molecular Weight: 60-71 kDa

Gene ID: 27102

UniProt: Q9BQI3

Pathways: Hepatitis C