EIF3I antibody

Catalog No. ABIN7113956

The Rabbit Polyclonal anti-EIF3I antibody has been validated for WB, ELISA, IHC, IF, IP and FACS. It is suitable to detect EIF3I in samples from Human, Mouse and Rat.

Quick Overview for EIF3I antibody (ABIN7113956)

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-EIF3I Antibody

Purpose: EIF3I antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit I

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF3I

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Alternative Name: EIF3I

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit I (eIF3i)|Eukaryotic translation initiation factor 3 subunit 2|TGF-beta receptor-interacting protein 1 (TRIP-1)|eIF-3-beta|eIF3 p36|EIF3I|EIF3S2|TRIP1

Background: Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 36 kDa

Gene ID: 8668

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA