GPI antibody

Catalog No. ABIN7114586

The Rabbit Polyclonal anti-GPI antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect GPI in samples from Human, Mouse and Rat.

Quick Overview for GPI antibody (ABIN7114586)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GPI Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: glucose phosphate isomerase

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3 ,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI

Background: Synonyms:AMF, Autocrine motility factor, g6pi, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36 Background:This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.

Molecular Weight: 56 kDa

Gene ID: 2821

UniProt: P06744