VEGFA antibody (AA 141-180)

Catalog No. ABIN707186

The Rabbit Polyclonal anti-VEGFA antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect VEGFA in samples from Human, Rat, Mouse and Chicken. There are 6+ publications available.

Quick Overview for VEGFA antibody (AA 141-180) (ABIN707186)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human, Rat, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-VEGFA Antibody

Binding Specificity: AA 141-180

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VEGF-A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-VEGFA antibody (ABIN707186)

Fetoni, Eramo, Paciello, Rolesi, Samengo, Paludetti, Troiani, Pani: "The redox protein p66(shc) mediates cochlear vascular dysfunction and transient noise-induced hearing loss." in: Scientific reports, Vol. 6, pp. 25450, (2016) (PubMed).

Shi, Ko, Ko, Kim, Ko: "Peptide Lv augments L-type voltage-gated calcium channels through vascular endothelial growth factor receptor 2 (VEGFR2) signaling." in: Biochimica et biophysica acta, Vol. 1853, Issue 5, pp. 1154-64, (2015) (PubMed).

Wang, Liao, Wang, Deng, Yu: "Transplantation of bone marrow stromal cells overexpressing human vascular endothelial growth factor 165 enhances tissue repair in a rat model of radiation-induced injury." in: Chinese medical journal, Vol. 127, Issue 6, pp. 1093-9, (2014) (PubMed).

Fetoni, Lattanzi, Eramo, Barba, Paciello, Moriconi, Rolesi, Michetti, Troiani, Paludetti: "Grafting and early expression of growth factors from adipose-derived stem cells transplanted into the cochlea, in a Guinea pig model of acoustic trauma." in: Frontiers in cellular neuroscience, Vol. 8, pp. 334, (2014) (PubMed).

Tian, Yang, Xiao, Huang, He, Ma, Xia, Shi: "Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model." in: The Journal of biological chemistry, Vol. 288, Issue 22, pp. 15988-6003, (2013) (PubMed).

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background:

Synonyms: VPF, VEGF, MVCD1, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VEGFA

Background: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling