HACE1 antibody

Catalog No. ABIN7114916

The Rabbit Polyclonal anti-HACE1 antibody is suitable to detect HACE1 in samples from Human. It has been validated for ELISA, WB and IF.

Quick Overview for HACE1 antibody (ABIN7114916)

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HACE1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HACE1 Antibody

Purpose: HACE1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for HACE1

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Alternative Name: HACE1

Background:

Synonyms: E3 ubiquitin-protein ligase HACE1|HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1|HECT-type E3 ubiquitin transferase HACE1|HACE1|KIAA1320

Background: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

Molecular Weight: 78 kDa

Gene ID: 57531

UniProt: Q8IYU2