IDH1 antibody (AA 120-414)

Catalog No. ABIN7115525

The Mouse Monoclonal anti-IDH1 antibody is suitable to detect IDH1 in samples from Human. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for IDH1 antibody (AA 120-414) (ABIN7115525)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 1G10

Product Details anti-IDH1 Antibody

Binding Specificity: AA 120-414

Purpose: IDH1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 120-414aa

Immunogen: isocitrate dehydrogenase 1(NADP+), soluble

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background:

Synonyms: Isocitrate dehydrogenase [NADP] cytoplasmic (IDH, IDH1)|Cytosolic NADP-isocitrate dehydrogenase|IDPc|NADP(+)-specific ICDH|Oxalosuccinate decarboxylase|IDH1|PICD

Background: IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25 % each wild type and mutant homo-dimers and 50 % hetero-dimers present in the tumor cells(PMID:21079649 ).

Molecular Weight: 46 kDa

Gene ID: 3417

UniProt: O75874

Pathways: Warburg Effect