MSH6 antibody

Catalog No. ABIN7116545

The Mouse Monoclonal anti-MSH6 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect MSH6 in samples from Human.

Quick Overview for MSH6 antibody (ABIN7116545)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: 4C4

Product Details anti-MSH6 Antibody

Purpose: MSH6 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: mutS homolog 6

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background:

Synonyms: DNA mismatch repair protein Msh6 (hMSH6)|G/T mismatch-binding protein (GTBP, GTMBP)|MutS protein homolog 6|MutS-alpha 160 kDa subunit (p160)|MSH6|GTBP

Background: Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3(H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Molecular Weight: 160 kDa

Gene ID: 2956

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response