Collagen IV antibody (AA 1571-1669)

Catalog No. ABIN707396

The Rabbit Polyclonal anti-Collagen IV antibody has been validated for WB, ELISA, IHC (fro), IHC (p), FACS, IF (p) and IF (cc). It is suitable to detect Collagen IV in samples from Human, Mouse, Rat and Pig. There are 2+ publications available.

Quick Overview for Collagen IV antibody (AA 1571-1669) (ABIN707396)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Collagen IV Antibody

Binding Specificity: AA 1571-1669

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Collagen IV antibody (ABIN707396)

Sato, Coburn: "Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells." in: PLoS neglected tropical diseases, Vol. 11, Issue 7, pp. e0005830, (2017) (PubMed).

Wu, Zhang, Ma, Zhang, Qin: "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." in: Molecular biology reports, Vol. 39, Issue 9, pp. 9085-93, (2012) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen 4

Background:

Synonyms: ICH, HANAC, POREN1, arresten, Collagen alpha-1(IV) chain, COL4A1

Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

Gene ID: 1282

UniProt: P02462