NSUN2 antibody

Catalog No. ABIN7116925

This Rabbit Polyclonal antibody specifically detects NSUN2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for NSUN2 antibody (ABIN7116925)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NSUN2 Antibody

Purpose: NSUN2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: NOL1/NOP2/Sun domain family, member 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2

Background:

Synonyms: RNA cytosine C(5)-methyltransferase NSUN2|Myc-induced SUN domain-containing protein (Misu)|NOL1/NOP2/Sun domain family member 2|Substrate of AIM1/Aurora kinase B|mRNA cytosine C(5)-methyltransferase|tRNA cytosine C(5)-methyltransferase|tRNA methyltransferase 4 homolog (hTrm4)|NSUN2|SAKI|TRM4

Background: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine(m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Molecular Weight: 90-100 kDa

Gene ID: 54888

UniProt: Q08J23