NRF2 antibody (AA 120-413)

Catalog No. ABIN7116949

This Rabbit Polyclonal antibody specifically detects NRF2 in WB, IHC, ELISA, IP and FACS. It exhibits reactivity toward Human, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for NRF2 antibody (AA 120-413) (ABIN7116949)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-NRF2 Antibody

Binding Specificity: AA 120-413

Purpose: NFE2L2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 120-413aa

Immunogen: nuclear factor(erythroid-derived 2)-like 2

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:1000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: NFE2L2

Background:

Synonyms: Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2, NFE2-related factor 2, Nrf-2)|HEBP1|Nuclear factor, erythroid derived 2, like 2|NFE2L2|NRF2

Background: NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response(ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress(PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC.

Molecular Weight: 70-100 kDa

Gene ID: 4780

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling