PAK4 antibody

Catalog No. ABIN7117185

This anti-PAK4 antibody is a Rabbit Polyclonal antibody detecting PAK4 in WB, ELISA, IHC, IF, FACS and IP. Suitable for Human, Mouse and Rat.

Quick Overview for PAK4 antibody (ABIN7117185)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-PAK4 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: p21 protein(Cdc42/Rac)-activated kinase 4

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background: Synonyms:KIAA1142, p21 activated kinase 4, PAK 4, PAK4 Background:Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Molecular Weight: 64 kDa

Gene ID: 10298

UniProt: O96013

Pathways: RTK Signaling