PIWIL2 antibody

Catalog No. ABIN7117541

The Rabbit Polyclonal anti-PIWIL2 antibody has been validated for IHC and ELISA. It is suitable to detect PIWIL2 in samples from Human, Mouse and Rat.

Quick Overview for PIWIL2 antibody (ABIN7117541)

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-PIWIL2 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: piwi-like 2(Drosophila)

Isotype: IgG

Application Details

Application Notes: IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PIWIL2

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Alternative Name: PIWIL2

Background: Synonyms:HILI Background:Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8(By similarity). Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors(PubMed:16377660).

Gene ID: 55124

UniProt: Q8TC59

Pathways: Stem Cell Maintenance