PUF60 antibody

Catalog No. ABIN7117635

The Rabbit Polyclonal anti-PUF60 antibody (ABIN7117635) specifically detects PUF60 in WB, IHC, ELISA, IP and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PUF60 antibody (ABIN7117635)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-PUF60 Antibody

Purpose: PUF60 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: poly-U binding splicing factor 60KDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100, IP: 1:20 - 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: PUF60

Background:

Synonyms: Poly(U)-binding-splicing factor PUF60|60 kDa poly(U)-binding-splicing factor|FUSE-binding protein-interacting repressor (FBP-interacting repressor)|Ro-binding protein 1 (RoBP1)|Siah-binding protein 1 (Siah-BP1)|PUF60|FIR|ROBPI|SIAHBP1

Background: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 65 kDa

Gene ID: 22827

UniProt: Q9UHX1