POLK antibody

Catalog No. ABIN7117655

The Rabbit Polyclonal anti-POLK antibody is suitable to detect POLK in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for POLK antibody (ABIN7117655)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-POLK Antibody

Purpose: POLK antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: polymerase(DNA directed) kappa

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK

Background:

Synonyms: DNA polymerase kappa|DINB protein (DINP)|POLK|DINB1

Background: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Molecular Weight: 70 kDa

Gene ID: 51426

UniProt: Q9UBT6

Pathways: DNA Damage Repair