POLR2F antibody

Catalog No. ABIN7117674

This anti-POLR2F antibody is a Rabbit Polyclonal antibody detecting POLR2F in WB, IF, ELISA and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for POLR2F antibody (ABIN7117674)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC)

Product Details anti-POLR2F Antibody

Purpose: POLR2F antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: polymerase(RNA) II(DNA directed) polypeptide F

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: POLR2F

Background:

Synonyms: DNA-directed RNA polymerases I, II, and III subunit RPABC2 (RNA polymerases I, II, and III subunit ABC2)|DNA-directed RNA polymerase II subunit F|DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide|RPABC14.4 (RPB14.4)|RPB6 homolog|RPC15|POLR2F|POLRF

Background: DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds(By similarity).

Molecular Weight: 14 kDa

Gene ID: 5435

UniProt: P61218

Pathways: Regulatory RNA Pathways