RAB5 antibody

Catalog No. ABIN7118228

The Rabbit Polyclonal anti-RAB5 antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect RAB5 in samples from Human, Mouse and Rat.

Quick Overview for RAB5 antibody (ABIN7118228)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RAB5 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: RAB5A, member RAS oncogene family

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP:1:200-1:2000, IHC: 1:50-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A

Background: Synonyms:RAB5, RAB5A, Ras related protein Rab 5A Background:The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes(PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension(PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan(PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3(By similarity).

Molecular Weight: 24 kDa

Gene ID: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity